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| # | Name | Duration | Finished At | Stats |
|---|---|---|---|---|
| 1 | Toad Circuit | 00:01:50 | 00:01:50 | |
| 2 | Daisy Hills | 00:02:34 | 00:04:25 | |
| 3 | Cheep Cheep Lagoon | 00:02:42 | 00:07:07 | |
| 4 | Shy Guy Bazaar | 00:02:50 | 00:09:58 | |
| 5 | Wuhu Loop | 00:02:31 | 00:12:29 | |
| 6 | Mario Circuit | 00:03:02 | 00:15:32 | |
| 7 | Music Park | 00:02:43 | 00:18:16 | |
| 8 | Rock Rock Mountain | 00:02:59 | 00:21:15 | |
| 9 | Piranha Plant Slide | 00:03:09 | 00:24:25 | |
| 10 | Wario Shipyard | 00:02:48 | 00:27:14 | |
| 11 | Neo Bowser City | 00:02:33 | 00:29:47 | |
| 12 | Maka Wuhu | 00:01:50 | 00:31:37 | |
| 13 | DK Jungle | 00:03:15 | 00:34:53 | |
| 14 | Rosalina’s Ice World | 00:02:42 | 00:37:35 | |
| 15 | Bowser’s Castle | 00:03:00 | 00:40:36 | |
| 16 | Rainbow Road | 00:02:25 | 00:43:02 | |
| 17 | N64 Luigi Raceway | 00:03:10 | 00:46:12 | |
| 18 | GBA Bowser Castle 1 | 00:01:53 | 00:48:05 | |
| 19 | Wii Mushroom Gorge | 00:02:24 | 00:50:30 | |
| 20 | DS Luigi’s Mansion | 00:02:41 | 00:53:11 | |
| 21 | N64 Koopa Beach | 00:02:48 | 00:55:59 | |
| 22 | SNES Mario Circuit 2 | 00:01:56 | 00:57:55 | |
| 23 | Wii Coconut Mall | 00:02:51 | 01:00:47 | |
| 24 | DS Waluigi Pinball | 00:03:16 | 01:04:04 | |
| 25 | N64 Kalimari Desert | 00:02:54 | 01:06:58 | |
| 26 | DS DK Pass | 00:02:57 | 01:09:55 | |
| 27 | GCN Daisy Cruiser | 00:02:20 | 01:12:16 | |
| 28 | Wii Maple Treeway | 00:03:16 | 01:15:32 | |
| 29 | Wii Koopa Cape | 00:03:48 | 01:19:21 | |
| 30 | GCN Dino Dino Jungle | 00:02:51 | 01:22:12 | |
| 31 | DS Airship Fortress | 00:02:53 | 01:25:05 | |
| 32 | SNES Rainbow Road | 00:01:52 | 01:26:58 |